Data/hora: 03/06/2024 08:10:14

Biblioteca(s): | Epagri-Sede.
Data corrente: | 30/10/2019
Data da última atualização: | 30/10/2019
Autoria: | PETRY, V. S.; STEFENON, V. M.; MACHADO, L. O.; KLABUNDE, G. H. F.; PEDROSA, F. O.; NODARI, R. O.
Título: | Repetitive genomic elements in Campomanesia xanthocarpa: prospection, characterization and cross amplifcation of molecular markers.
Ano de publicação: | 2019
Fonte/Imprenta: | 3 Biotech, Nova Iorque, v. 9, p. 1-11, 2019.
Idioma: | Inglês

Conteúdo:

Repetitive genomic elements were prospected in Campomanesia xanthocarpa, aiming to characterize these elements in a
non-model plant species and to develop species-specifc microsatellite markers. Approximately 4.12% of the partial genome
of C. xanthocarpa is composed of repetitive elements, being retrotransposons the most widely represented. A total of nine
polymorphic microsatellite markers were obtained: four nuclear-neutral, two nuclear EST, two plastidial and one mitochondrial.
Levels of population genetic diversity of four natural populations of C. xanthocarpa were characterized using these markers. In
addition, the cross-species amplifcation of the microsatellite markers was tested in seven species of tribe Myrteae (Myrtaceae).
The characterized microsatellite markers revealed low to moderate levels of genetic diversity (expected heterozygosity range:
0.33?0.57; observed heterozygosity: 0.26?0.74 and number of alleles: 2.25?4.25). Cross-species amplifcation was successful
for all loci, except Cxant76. These nine markers will contribute for studies on genetic diversity, gene fow, plant selection and
breeding of this species, towards the conservation of natural populations, as well as its commercial use.

Palavras-Chave: | Guabiroba; mtSSRs; nSSRs; ptSSRs; Transferability; Transposons.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_43731.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
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